Advanced Bacterial Photography: Opti coli
Thursday, February 02, 2023 at 1:00pm to 4:00pm
Huang-Hobbs BioMaker Space, 26-033 / 26-035 60 Vassar St, Cambridge, MA 02139
An extended format of the Bacterial Photography Workshop, users gain additional experience in design and the cloning process. In 6 sessions, students learn about the synthetic biology system, design the construction of a plasmid, complete the cloning process, integrate the plasmid into bacteria, and select for successful transformant, and create a photography plate.
Some wet lab experience recommended, but all experience levels welcome (optional micropipette training available). Advanced students may choose to implement independent design projects and make modifications to the existing system.
Session 1: Overview, Background, Design Session. Students will plan implmenentation for the remaining sessions and design primers for PCR amplification of target genes. Optional mircopipette session following.
Session 2: Plasmid Mini-Prep, PCR, and Restriction Digestion. Students will learn several routine techniques for working with recombinant DNA.
Session 3: Gel Electrophoresis. Students will characterize products from earlier sessions and prepare DNA for assembly.
Session 4: Assembly and Transformation. Students will assemble plasmid DNA and transform into a bacterial host.
Session 5: Colony Selection and Liquid Culture. Students will pick colonies from tranformation plate and start liquid culture.
Session 6: Photograph Plate. Students will create the agar petri dish with the engineered bacteria and produce a photograph of their selected image.
Attendence at all sessions is required. Participation limited to 8. Please complete the application on the registration link. (https://tinyurl.com/HHBMS-IAP2023)
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School of Architecture and Planning (SA+P), School of Engineering (SoE), School of Humanities, Arts, and Social Sciences (SHASS), School of Science
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