This is a Level 2 Certification Credential in the Huang-Hobbs BioMaker Space Credential Certification Program. Open to all trained users in the "biomakerspace" training group, and those who have completed the required pre-requisites trainings with a sufficient level of proficiency. Email jbuck@mit.edu to be added to the training group and for additional information about this program. Limited to 8 participants. To register, please visit http://tinyurl.com/HHBMS-IAP2024.

Participants will review the theory and practice of polymerase chain reaction for DNA amplification. Student will design primers to amplify various DNA fragments, will setup thermocycler programs, setup PCR reactions using master mixes, run PCR reactions, and characterize results. Students will be assessed on proficiency.

Pre-Requisite Trainings: Agarose Gel Electrophoresis (Level 1), DNA Isolation & Purification (Level 1)