This is a Level 2 Certification Credential in the Huang-Hobbs BioMaker Space Credential Certification Program. Open to all trained users in the "biomakerspace" training group, and those who have completed the required pre-requisites trainings with a sufficient level of proficiency. Email jbuck@mit.edu to be added to the training group and for additional information about this program. Limited to 6 participants. To register, please visit http://tinyurl.com/HHBMS-IAP2024.

Participants will review the theory and practice of mammalian cell culture. Participants will gain experience working with suspended (TK6) and adherent cells (HeLa or 3T3) in a biosafety cabinet using standard BL2 practices. Students will learn media changing and passage cells to maintain growth conditions and will use trypsin treatments to release cells from the culture flask. Students will learn basic use of an inverted light mircoscope and cell counting using a hemacytometer and trypan blue stain. Students be assessed on proficiency.

Pre-Requisite Trainings: Microbial Culturing & Sterile Technique (Level 1)

Two-Session Training (PARTICIPANTS ARE REQUIRED TO ATTEND BOTH SESSIONS)