This is a Level 1 Certification Credential in the Huang-Hobbs BioMaker Space Credential Certification Program. Open to all trained users in the "biomakerspace" training group, and those who have completed the required pre-requisites trainings with a sufficient level of proficiency. Email jbuck@mit.edu to be added to the training group and for additional information about this program. Limited to 20 participants. To register, please visit http://tinyurl.com/HHBMS-IAP2024.

Participants will review the basics of extracting plasmid and genomic DNA from cells, and purifying the recovered DNA. Participants will use various buffer and disruption techniques to lyse cells. Participants will learn use of columns to and alcohol precipitation to purify extracted DNA. Participants will quantify DNA using UV spectroscopy on a micro-volume UV/Vis spectrophotometer.  Students will be assessed on proficiency.

Pre-Requisite Trainings: Pipette and Vis Spec (Level 0), Lab Math and Solutions (Level 0),

Recommend Trainings: Microbial Culture (Level 1)