This is a Level 2 Certification Credential in the Huang-Hobbs BioMaker Space Credential Certification Program. Open to all trained users in the "biomakerspace" training group, and those who have completed the required pre-requisites trainings with a sufficient level of proficiency. Email jbuck@mit.edu to be added to the training group and for additional information about this program. Limited to 12 participants. To register, please visit http://tinyurl.com/HHBMS-IAP2024.

Participants will review the theory and practice of microbial transformation. Students will utilize electroporation to transfer vector DNA into E. coli host cells, as well heat shock methods of transformation. Advanced students will explore additional methods of transformation for yeast (e.g. Lithium Acetate) and bacterial conjugation. Students will determine transformation efficiencies. Students will be assessed on proficiency.

Pre-Requisite Trainings: Microbial Culturing & Sterile Technique (Level 1)

Recommend Training: Agarose Gel Electrophoresis (Level 1), DNA Isolation & Purification (Level 1)