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X-WR-CALNAME:MechE Colloquium 
X-WR-TIMEZONE:Eastern Time (US & Canada)
BEGIN:VEVENT
DTSTAMP:20261017T160752Z
UID:tag:localist.com\,2008:EventInstance_44207037205128
DTSTART:20230915T193000Z
DTEND:20230915T203000Z
DESCRIPTION:Professor Cullen Buie\n\nAbstract: \n \nIn this talk we will di
 scuss work in our laboratory to use electric fields coupled with fluid flo
 w in microfluidic devices to enable high throughput delivery of nucleic ac
 ids to cells. Conventional electroporation approaches for bacterial gene d
 elivery have distinct advantages\, but they are typically limited to relat
 ively small sample volumes\, reducing their utility for applications requi
 ring high throughput such as the generation of mutant libraries. Here\, we
  present a scalable\, large-scale bacterial gene delivery approach enabled
  by a disposable\, user-friendly microfluidic electroporation device requi
 ring minimal fabrication. We demonstrate that the proposed device can outp
 erform conventional cuvettes in a range of situations\, including across E
 scherichia coli strains with a range of electroporation efficiencies\, and
  we use its large-volume bacterial electroporation capability to generate 
 a library of transposon mutants in the anaerobic gut commensal Bifidobacte
 rium longum. Results of this work hold exciting promise for accelerating g
 enetic engineering of bacteria with wide ranging applications in industry 
 and healthcare. Further\, we will present recent efforts by a company spun
  out of the Buie Laboratory\, Kytopen\, which is leveraging the electropor
 ation work to enable scalable non-viral transfection of mammalian cells. A
 pplications of this work include engineered cell therapies such as CAR-T\,
  which are currently plagued by high costs and manufacturing issues. The n
 on-viral transfection approach developed by Kytopen has the potential to s
 implify and accelerate the development of life saving cellular therapies.
LOCATION:Building 3\, Room 270
SUMMARY:MechE Colloquium 
URL;VALUE=URI:https://calendar.mit.edu/event/meche_colloquium
CATEGORIES:Conferences/Seminars/Lectures
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